News briefing： The purpose of this study was to detect the presence of PPRV (Small Ruminant Disease Virus) in Assam goats.

The purpose of this study was to detect the presence of PPRV (Small Ruminant Disease Virus) in Assam goats. Competitive ELISA and double antibody sandwich ELISA were used to detect PPR virus antibody and antigen. In addition, this study also included the use of RT-PCR technology to evaluate and detect PPRV specific target genes in clinical samples. A total of 579 serum samples (68.65% were sudden samples and 5.29% were random samples) were collected from different areas of Assam for competitive enzyme-linked immunosorbent assay (c-ELISA), indicating that the total prevalence rate of goats was 27.28%.

Goat serum samples with PPRV antibody detected were collected from different areas of Assam during the outbreak. The percentages of Kamrup, Nalbari, Mongoldoi, Jorhat, Darrang and Barpeta areas were 79.26%, 85.41%, 58.82%, 6%, 29.41% and 36.36% respectively. However, a high prevalence rate (20.83%) was observed in the Dhubri area where random samples were collected. Among the suspected samples, a high prevalence rate (85.41%) was observed in Nalbari area. The competitive percentage values (ranging from 35 to 45) of goat samples detected by competitive ELISA can be divided into three groups: positive, suspected and negative.

Most serum samples (n=158) with competition percentage less than or equal to 35% have PPRV antibodies, which are considered positive. When (n=9) is greater than 35% and less than or equal to 45%, they are considered suspicious and need to be retested. When (n=423) is greater than 45%, they are considered negative. The total sensitivity, specificity, apparent prevalence and true prevalence were 68.65%, 94.70%, 27.28% and 34.69%, respectively. In this study, the true prevalence rate was calculated according to the sensitivity and specificity of c-ELISA, with a relative specificity of 94.70% and a sensitivity of 68.65%.